化学生物学 Chemical Biology
颜晓梅课题组: 生化分析与生物传感

Xiao-Mei YAN — Biochemical Analysis and Biosensing

Group Members
Associate Professor Lina Wu (吴丽娜)

Selected Publications

  1. Yu, MX; Wu, LN; Huang, TX; Wang, S; Yan, XM*. Rapid detection and enumeration of total bacteria in drinking water and tea beverages by a laboratory-built high-sensitivity flow cytometer, Anal. Methods, 2015, 7, 3072-3079.
  2. Zhang, Z*; Deng, CQ; Meng, LS; Zheng, Y; Yan, XM. A rhodamine hydrazide-based fluorescent probe for sensitive and selective detection of hypochlorous acid and its application in living cells, Anal. Methods, 2015, 7, 107-114.
  3. Zhu, SB; Ma, L; Wang, S; Chen, CX; Zhang, WQ; Yang, LL; Hang, W; Nolan, J. P.; Wu, LN; Yan, XM*. Light-scattering detection below the level of single fluorescent molecules for high-resolution characterization of functional nanoparticles. ACS Nano, 2014, 8, 10998-11006.
  4. Zhang, X; Zhang, SY; Zhu, SB; Chen, S; Han, JY; Gao, KM; Zeng, JZ; Yan, XM*. Rapid identification of mitochondria-targeting anticancer compounds by an in vitro strategy, Anal. Chem., 2014, 86, 5232?5237.
  5. Wu, LN; Luan, T; Yang, XT; Wang, S; Zheng, Y; Huang, TX; Zhu, SB; Yan, XM*. Trace detection of specific viable bacteria using tetracysteine-tagged bacteriophages, Anal. Chem., 2014, 86, 907-912.
  6. Yang, CW; Chen, S; Zhou, M; Li, Y; Li, YF; Zhang, ZX; Liu, Z; Ba, Q; Li, JQ; Wang, H; Yan, XM*; Ma, DW*; Wang, RX*. Development of 3-Phenyl-N-(2-(3-phenylureido) ethyl)-thiophene-2-sulfonamide compounds as inhibitors of antiapoptotic Bcl-2 family proteins, ChemMedChem, 2014, 9, 1436 - 1452.
  7. Xu, F; Zhu, L; Zhu, SB; Yan, XM; Xu, HC*. Electrochemical intramolecular aminooxygenation of unactivated alkenes, Chem. Eur. J., 2014, 20, 12740- 12744.
  8. Huang, ZZ; Zhang, SD; Hang, W*; Chen, YD; Zheng, JX; Li, W; Xing, JC; Zhu, EY; Yan, XM. Liquid chromatography-mass spectrometry based serum peptidomic approach for renal clear cell carcinoma diagnosis, J. Pharmaceut. Biomed., 2014, 100, 175-183.
  9. Shao, Q; Zheng, Y; Dong, XM; Tang, K; Yan, XM*; Xing BG*. A covalent reporter of beta-lactamase activity for fluorescent imaging and rapid screening of antibiotic-resistant bacteria, Chem. Eur. J., 2013, 19, 10903-10910.
  10. Yang, LL;? Huang, TX;? Zhu, SB; Zhou YX; Jiang, YB; Wang, S; Chen, YQ; Wu, LN; Yan XM*. High-throughput single-cell analysis of low copy number beta-Galactosidase by a laboratory-built high-sensitivity flow cytometer, Biosens. Bioelectron., 2013, 48, 49-56.
  11. Chen, CX; Zhu, SB; Huang, TX; Wang S; Yan, XM*. Analytical techniques for single-liposome characterization. Anal. Methods, 2013, 5, 2150-2157.
  12. Yang, L; Wu, SQ; Lin, BJ; Huang, TX; Chen, XP; Yan, XM; Han, SF*. A targetable nanogenerator of nitric oxide for light-triggered cytotoxicity. J. Mater. Chem. B, 2013, 1, 6115.
  13. Huang, ZZ; Chen, YJ; Hang, W.*, Gao, Y.; Lin, L.; Li, D. Y.; Xing, JC; Yan, XM. Holistic Metabonomic Profiling of Urine Affords Potential Early Diagnosis for Bladder and Kidney Cancers, Metabolomics, 2013, 9, 119-129.
  14. Zhang, SY; Zhu, SB; Yang, LL; Zheng, Y; Gao, M; Wang, S; Zeng, J-Z; Yan, XM*. High-Throughput Multiparameter Analysis of Individual Mitochondria, Anal. Chem., 2012, 84, 6421-6428.
  15. Yang, LL; Zhou, YX; Zhu, SB; Huang, TX; Wu, LN; Yan, XM*. Detection and quantification of bacterial autofluorescence at the single-cell level by a laboratory-built high-sensitivity flow cytometer, Anal. Chem., 2012, 84, 1526-1532.
  16. Huang, H; Chen, ZJ; Yan, XM*. Simultaneous determination of serotonin and creatinine in urine by combining two ultrasound-assisted emulsification microextractions with on-column stacking in capillary electrophoresis, J. Sep. Sci., 2012, 35, 436-444.
  17. Lin, L; Huang, ZZ; Gao, Y; Chen, YJ; Hang, W.*, Xing, JC; Yan, XM. LC-MS based serum metabolic profiling for genitourinary cancer classification and cancer type-specific biomarker discovery, Proteomics, 2012, 12, 2238-2246.
  18. Lu, N.; Liu, JX; Liu, J.; Zhang, CY; Jiang, FQ; Wu, H.; Chen, LQ; Zeng, WJ; Cao, XH; Yan, TD; Wang, GH; Zhou, H.; Lin, BZ; Yan, XM; Zhang, XK; and Zeng, JZ*. Antagonist effect of triptolide on AKT activation by truncated retinoid X receptor-alpha. PLoS One 2012, 7(4): e35722.
  19. Gao, Y.; Yang, N.; Yan, XM; Hang, W.*; Xing, JC; Zheng, J.; Zhu, EY; Huang, BL. Early diagnosis of urinary lithiasis via elementary profile of serum samples, Anal. Methods, 2012, 4, 693-698.
  20. Wu, LN; Huang, TT; Yang, LL; Pan, JB; Zhu, SB; Yan, XM*. Sensitive and selective bacterial detection using tetracysteine-tagged phages in conjunction with biarsenical dye, Angew. Chem. Int. Ed., 2011, 50, 5873-5877.
  21. Zhu, SB; Wang, S; Yang, LL; Huang, TX; Yan, XM*. Progress in the development of techniques based on light scattering for single nanoparticle detection, Sci. China Chem., 2011, 54, 1244-1253.
  22. Zheng, Y; Lin, L; Hang, W; Yan, XM*; Marrone, B. L.* Analysis of beryllium to biomolecule binding using a metal specific fluorescent probe and competitive Assay, Talanta, 2011, 85, 638-643.
  23. Zhang, Z*; Zheng, Y; Hang, W; Yan, XM*; Zhao, YF. Sensitive and selective off-on rhodamine hydrazide fluorescent chemosensor for hypochlorous acid detection and bioimaging, Talanta, 2011, 85, 779-786.
  24. Huang, ZZ; Lin, L; Gao, Y; Chen, YJ; Yan, XM; Xing, JC; Hang, W*. Bladder cancer determination via two urinary metabolites: a biomarker pattern approach, Mol. Cell. Proteomics, 2011, 10, 10.1074/mcp.M111.007922-1–007922-10.
  25. Lin, L; Huang, ZZ; Gao, Y; Yan, XM; Xing, JC; Hang, W*. LC-MS based serum metabonomic analysis for renal cell carcinoma diagnosis, staging, and biomarker discovery, J. Proteome. Res., 2011, 10, 1396-1405.
  26. Zhu, SB; Yang, LL; Long, Y; Gao, M; Huang, TX; Hang, W; Yan, XM*. Size differentiation and absolute quantification of gold nanoparticles via single particle detection with a laboratory-built high-sensitivity flow Cytometer, J. Am. Chem. Soc., 2010, 132, 12176-12178.
  27. Yang, LL; Wu, LN; Zhu, SB; Long, Y; Hang, W; Yan, XM*. Rapid, absolute, and simultaneous quantification of specific pathogenic strain and total bacterial cells using an ultrasensitive dual-color flow Cytometer, Anal. Chem., 2010, 82, 1109-1116.
  28. Zhang, ZL; Long, Y; Pan, JB; Yan, XM*. Preparation of fluorescence-encoded microspheres in a core-shell structure for suspension arrays, J. Mater. Chem., 2010, 20, 1179-1185.
  29. Long, Y; Zhang, ZL; Yan, XM*; Xing, JC*; Zhang, KY; Huang, JX; Zheng, JX; Li, W. Multiplex immunodetection of tumor markers with a suspension array built upon core-shell structured functional fluorescence-encoded microspheres, Anal. Chim. Acta, 2010, 665, 63-68.
  30. Yan, XM; Lin, YM; Huang, RF; Hang, W*; Harrison, W. W. A spectroscopic investigation of the afterglow and recombination process in a microsecond pulsed glow discharge, J. Anal. At. Spectrom., 2010, 25, 534-543.
  31. Lin, L; Yu, Q; Yan, XM; Hang, W*; Zheng, JX; Xing, JC; Huang, BL. Direct infusion mass spectrometry or liquid chromatography mass spectrometry for human metabonomics? A serum metabonomic study of kidney cancer, Analyst, 2010, 135, 2970-2978.
  32. Xing, JC*; Yan, LJ; Zhang, J*; Lin, L; Gao, Y; Chen, WG; Song, XY; Yan, XM; Hang, W*; Huang, BL. A comparative study of elution gradients in UPLC-TOF-MS-based metabonomics research, Chromatographia, 2010, 72, 807-813.
  33. Yang, LL; Zhu, SB; Hang, W; Wu, LN; Yan, XM*. Development of an ultrasensitive dual-channel flow cytometer for the individual analysis of nanosized particles and biomolecules, Anal. Chem., 2009, 81, 2555-2563.
  34. Ding, J; Lin, L; Hang, W; Yan, XM*. Beryllium uptake and related biological effects studied in THP-1 differentiated macrophages, Metallomics, 2009, 1, 471-478.
  35. Chen, ZJ; Yan, XM*. Simultaneous determination of melamine and 5-hydroxymethylfurfural in milk by capillary electrophoresis with diode array detection, J. Agric. Food Chem., 2009, 57, 8742-8747.
  36. Zhang, J*; Yan, LJ; Chen, WG; Lin, L; Song, XY; Yan, XM; Hang, W*; Huang, BL. Metabonomics research of diabetic nephropathy and type 2 diabetes mellitus based on UPLC-oaTOF-MS system, Anal. Chim. Acta, 2009, 650, 16-22.
  37. Chen, LZ; Lin, L; Yu, Q; Yan, XM; Hang, W*; He, J; Huang, BL. Semiquantitative multielemental analysis of biological samples by a laser ionization orthogonal time-of-flight mass spectrometer, J. Am. Soc. Mass Spectrom., 2009, 20, 1355-1358.
  38. Yang, LL; Zhu, SB; Hang, W; Yan, XM*. Development of a single-molecule flow analyzer, Chem. J. Chinese U., 2008, 29, 1549-1551.
  39. Zhang, J*; Yan, LJ; Lin, L; Chen, WG; Song, XY; Yan, XM; Hang, W*; Huang, BL. Metabonomics research of diabetic mellitus and diabetic nephropathy based on UPLC-oaTOF-MS system, Chem. J. Chinese U., 2008, 29, 2171-2173.
  40. He, J; Zhong, WW; Tang, AJ; Yan, XM; Lewis, C.; Majidi, V.; Hang, W. A fluorescence detection scheme for ultra large molecules after gas phase separation, Talanta, 2007, 71, 2126-2128.
  41. Yan, XM*; Zhong, WW; Tang, AJ; Schielke, E. G.; Hang W, Nolan J. P. Multiplexed flow cytometric immunoassay for influenza virus detection and differentiation, Anal. Chem., 2005, 77, 7673-7678.
  42. Yan, XM*; Habbersett R. C.; Yoshida, T. M.; Nolan, J. P.; Jett, J. H.; Marrone, B. L. Probing the kinetics of SYTOX Orange stain binding to double-stranded DNA with implications for DNA analysis, Anal. Chem., 2005, 77, 3554-3562.

授权国家发明专利

  • 颜晓梅,杨玲玲, 张洁,朱少彬. 生物流式分析仪. 国家发明专利, 授权专利,专利号:ZL 200810070842.9.
  • 颜晓梅, 张志灵, 潘建波,许艳军. 羧基化荧光编码微球及其合成方法. 国家发明专利, 授权专利,专利号:ZL 200810072052.4.

颜晓梅 教授、博士生导师

 
博士(厦门大学化学系,1996)
学士(厦门大学科仪系,1990)
日本名古屋大学联合培养(1994-1996)
博士后(美国佛罗里达大学,1996-1997)
博士后(美国Los Alamos 国家实验室,1997-2001)
研究员(美国Los Alamos 国家实验室,2001-2005)
教授 (厦门大学,2005.8- )

2012年获得国家杰出青年科学基金


电话:(0592)2184519(办公室)
   (0592)2180337(实验室)
传真:(0592)2189959
电子邮箱:xmyan@xmu.edu.cn

研究兴趣:生物分析新技术与新方法:1)超高灵敏流式检测仪的研制及其在纳米颗粒表征、纳米生物、纳米医学、微生物检测、分子生物学及细胞生物学研究中的应用; 2)功能化纳米颗粒的合成及其生化分析、生物医学应用; 3)噬菌体在细菌鉴别及纳米生物研究中的应用;4)液相芯片技术的开发及其在食品安全、环境监测、生物医学中的应用; 5)蛋白-蛋白相互作用研究新方法。

 




Xiao-Mei YAN Professor

 
B.E.(1990), Xiamen University, China;
Ph.D.(1996), Xiamen University, China;
Post-doc (1996-1997), University of Florida, US;
Post-doc and technical staff member (1997-2001, 2001-2005), Los Alamos National Laboratory, US;
Professor (2005- ), Xiamen University.

Research Interests: Biochemical analysis and biosensing, mainly include: development of high sensitivity flow cytometry for applications in nano- biotechnology, nanomedicine, micro- organism detection, molecular and cellular biology; development of suspension array technology (liquid chip) for biomedical applications.

Honors and Awards:
National Science Fund for Distinguished Young Scholars (2012)