主要研究成果

近年来,在 NSFC、973、863 等科研基金的资助下,本研究小组针对典型污染元素和持久性有机污染物在环境介质中的含量和化学形态分析方法学以及植物抵御污染环境胁迫的调控机制,系统地展开了以色谱-原子光谱/质谱联用检测技术为主要手段的基础和应用基础研究。其中主要研究成果包括:

(1)革新了传统原子光谱/质谱进样方式和色谱联用接口技术。率先报道了纳米氧化钛光催化导带电子和巯基乙醇光诱导S.还原的光催化/光诱导化学蒸气发生新原理,并用于硒和汞的形态分析;发展了基于热扩散原理的程序升温式热扩散GC/ICP-MS接口新技术,并用于汞和铅多形态同时分析;发展了cGC/ECNI-MS的脉冲式大体积进样新技术及其耦合新方法,并用于典型卤代持久性有机污染物(H-POPs)的高灵敏准确分析;自主设计和研制了相关便携式分析仪器和部件,最大限度地释放分析仪器的检测能力,并成功应用于野外环境现场检测。

(2)发展和丰富了生物分子检测策略,提出并建立了以元素为标签的生物标志分子的原子光谱/元素质谱筛选和定量方法,应用于硒蛋白和汞标记蛋白的筛选和绝对定量分析。

(3)利用所发展的HPLC/ICP-qMS/ESI-IT-MS联用新技术,首次发现并鉴定了在体镉、汞诱导的植物螯合肽(PCs)配合物in-vivo PCs-Cd和PCs-Hg,给出了植物抵御细胞氧化机制中PCs络合钝化设想长期缺乏的实验证据。

(4)率先提出树木“时间隧道”和“自然内标”概念、发展了系统的分析检测方法,建立了大气与树木表皮间POPs分配模型,填补了我国大气POPs污染空间分布和历史演变数据的空白。

已发表论文

96 Counting and Recognizing Single Bacterial Cells by a Lanthanide-Encoding Inductively Coupled Plasma Mass Spectrometric Approach, Analytical Chemistry, 2019, Accepted 下载全文
95 Towards a high peak capacity of 130 using nanoflow hydrophilic interaction liquid chromatography, Analytica Chimica Acta, 2019, 1062: 147-155 下载全文
94 A novel double-modified strategy to enhance the performance of thin-film nanocomposite nanofiltration membranes: Incorporating functionalized graphenes into supporting and selective layers, Chemical Engineering Journal, 2019, 368: 186-201 下载全文
93 Inhibitory Covalent Labeling and Clickable-Eu-Tagging-Based ICPMS: Measurement of pH-Dependent Absolute Activities of the Cathepsins in Hepatocyte Lysosomes, Analytical Chemistry, 2019, 91(11): 7032-7038 下载全文
92 Viruslike Element-Tagged Nanoparticle Inductively Coupled Plasma Mass Spectrometry Signal Multiplier: Membrane Biomarker Mediated Cell Counting, Analytical Chemistry, 2019, 91(8): 4948-4952 下载全文
91 Electrochromatographic behavior of core-shell particles: A comparison study, Analytica Chimica Acta, 2018, 1033: 205-212 下载全文
90 Dual polyhedral oligomeric silsesquioxanes polymerization approach to mutually-mediated separation mechanisms of hybrid monolithic stationary and mobile phases towards small molecules, Journal of Chromatography A, 2018, 1533: 136-142 下载全文
89 Near-Infrared Neodymium Tag for Quantifying Targeted Biomarker and Counting Its Host Circulating Tumor Cells, Analytical Chemistry, 2017, 89(17): 9239-9246 下载全文
88 Step-by-Step Design and Synthesis of Au@SiO2@Phenyl-azathiacrown for SERS-Based Specific Quantification of Inorganic Mercury, ChemistryOpen, 2017, 6(2): 201-205 下载全文
87 Enhancing the performance of thin-film nanocomposite nanofiltration membranes using MAH-modified GO nanosheets, RSC Advances, 2017, 7(86): 54898-54910 下载全文
86 Preparation and characterization of novel alkali-resistant nanofiltration membranes with enhanced permeation and antifouling properties: the effects of functionalized graphene nanosheets, RSC Advances, 2017, 7(30): 18755-18764 下载全文
85 ICPMS-Based Specific Quantification of Phosphotyrosine: A Gallium Tagging and Tyrosine-Phosphatase Mediated Strategy, Analytical Chemistry, 2016, 88(20): 9890-9896 下载全文
84 Silica-based polypeptide-monolithic stationary phase for hydrophilic chromatography and chiral separation, Journal of Chromatography A, 2016, 1446: 125-133 下载全文
83 An ongoing path of element-labeling/tagging strategies toward quantitative bioanalysis using ICP-MS, Applied Spectroscopy Reviews, 2016, 51(2): 117-128 下载全文
82 A Newly Identified Passive Hyperaccumulator Eucalyptus grandis x E. urophylla under Manganese Stress, PLoS One, 2015, 10(9): e0136606 下载全文
81 Two dimensional separations of human urinary protein digest using a droplet-interfaced platform, Analytica Chimica Acta, 2015, 863: 86-94 下载全文
80 Quantification and visualization of glutathione S-transferase omega 1 in cells using inductively coupled plasma mass spectrometry (ICP-MS) and fluorescence microscopy, Analytical and Bioanalytical Chemistry, 2015, 407(9): 2373-2381 下载全文
79 Determination and speciation of Hg using HPLC-AFS by atomization of this metal on a UV/nano-ZrO2/HCOOH photocatalytic reduction unit, Journal of Analytical Atomic Spectrometry, 2015, 30(4): 916-921 下载全文
78 Towards high peak capacity separations in normal pressure nanoflow liquid chromatography using meter long packed capillary columns, Analytica Chimica Acta, 2014, 852: 267-273 下载全文
77 A comparison study of in-column and on-column detection for electrochromatography, Journal of Chromatography A, 2014, 1362: 225-230 下载全文
76 Evaluation of BDE-47 Hydroxylation Metabolic Pathways Based on a Strong Electron-Withdrawing Pentafluorobenzoyl Derivatization Gas Chromatography/Electron Capture Negative Ionization Quadrupole Mass Spectrometry, Environmental Science & Technology, 2014, 48(14): 8117-8126 下载全文
75 Fabrication and investigation of electrochromatographic columns with a simplex configuration, Journal of Chromatography A, 2014, 1349: 90-95 下载全文
74 EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells, Oncology Letters, 2014, 8(1): 41-46 下载全文
73 A chemical “hub” for absolute quantification of a targeted protein: orthogonal integration of elemental and molecular mass spectrometry, Chemical Communications, 2014, 50: 6578-6581 下载全文
72 Click Chemistry Mediated Eu-Tagging: Activity-Based Specific Quantification and Simultaneous Activity Evaluation of CYP3A4 Using Eu-153 Species-Unspecific Isotope Dilution Inductively Coupled Plasma Mass Spectrometry, Analytical Chemistry, 2014, 86(8): 3688-3692 下载全文
71 Toward rapid preparation of capillary columns for electrochromatography use, Electrophoresis, 2014, 35(6): 836-839 下载全文
70 A "plug-and-use" approach towards facile fabrication of capillary columns for high performance nanoflow liquid chromatography, Journal of Chromatography A, 2014, 1325: 109-114 下载全文
69 ICP-MS-Based Multiplex and Ultrasensitive Assay of Viruses with Lanthanide-Coded Biospecific Tagging and Amplification Strategies, Analytical Chemistry, 2013, 85(20): 9428-9432 下载全文
68 Detection and quantification of proteins and cells by use of elemental mass spectrometry: progress and challenges, Analytical and Bioanalytical Chemistry, 2013, 405(17): 5663-5670 下载全文
67 Chemical interactions of mercury species and some transition and noble metals towards metallothionein (Zn7MT-2) evaluated using SEC/ICP-MS, RP-HPLC/ESI-MS and MALDI-TOF-MS, Metallomics, 2013, 5(7): 855-860 下载全文
66 Performance of single particle fritted capillary columns in electrochromatography, Journal of Chromatography A, 2013, 1272: 136-140 下载全文
65 Studies of biouptake and transformation of mercury by a typical unicellular diatom Phaeodactylum tricornutum, Chinese Science Bulletin, 2013, 58(2): 256-265 下载全文
64 Synthesis and characterization of a high selective mercury(II)-imprinted polymer using novel aminothiol monomer, Talanta, 2012, 101: 261-266 下载全文
63 Integrin-Targeted Trifunctional Probe for Cancer Cells: A "Seeing and Counting" Approach, Analytical Chemistry, 2012, 84(21): 8946-8951 下载全文
62 A Way to Probe the Microenvironment of Free Sulfhydryls in Intact Proteins with a Series of Monofunctional Organic Mercurials, Chemistry-A European Journal, 2012, 18(44): 13989-13993 下载全文
61 Europium-Labeled Activity-Based Probe through Click Chemistry: Absolute Serine Protease Quantification Using 153Eu Isotope Dilution ICP/MS, Angewandte Chemie-International Edition, 2012, 51(14): 3358-3363 下载全文
60 Nanosemiconductor-Based Photocatalytic Vapor Generation Systems for Subsequent Selenium Determination and Speciation with Atomic Fluorescence Spectrometry and Inductively Coupled Plasma Mass Spectrometry, Analytical Chemistry, 2012, 84(6): 2974-2981 下载全文
59 "One-pot" preparation of basic amino acid-silica hybrid monolithic column for capillary electrochromatography, Journal of Separation Science, 2011, 34(16-17): SI2314-2322 下载全文
58 A dual-labelling strategy for integrated ICPMS and LIF for the determination of peptides, Journal of Analytical Atomic Spectrometry, 2011, 26(6): 1175-1177 下载全文
57 Mechanisms of chemical generation of volatile hydrides for trace element determination (IUPAC Technical Report), Pure and Applied Chemistry, 2011, 83(6): 1283-1340 下载全文
56 Lanthanide-Coded Protease-Specific Peptide–Nanoparticle Probes for a Label-Free Multiplex Protease Assay Using Element Mass Spectrometry: A Proof-of-Concept Study, Angewandte Chemie-International Edition, 2011, 50(22): 5130-5133 下载全文
55 Strategies for Protein Quantification by Integrated Elemental and Molecular Mass Spectrometry, Clinical Chemistry and Laboratory Medicine, 2011, 49: S137 下载全文
54 Evaluation of cadmium species lability using ion-pair reversed phase HPLC coupled on-line with inductively coupled plasma mass spectrometry, Talanta, 2011, 84(2): 287-292 下载全文
53 Free cadmium ions released from CdTe-based nanoparticles and their cytotoxicity on Phaeodactylum tricornutum, Metallomics, 2010, 2(7): 469-473 下载全文
52 SE-HPLC/ICP-MS/ESI-MS联用技术用于富镉植物中镉的形态研究, 光谱学与光谱分析, 2010, 30(4): 1096-1100 下载全文
51 Dynamic Labeling Strategy with Hg-204-Isotopic Methylmercurithiosalicylate for Absolute Peptide and Protein Quantification, Analytical Chemistry, 2010, 82(5): 1616-1620 下载全文
50 Absolute Quantification of Intact Proteins via 1,4,7,10-Tetraazacyclododecane-1,4,7-trisacetic acid-10-Maleimidoethylacetamide-Europium Labeling and HPLC Coupled with Species-Unspecific Isotope Dilution ICPMS, Analytical Chemistry, 2010, 82(4): 1261-1269 下载全文
49 Bioaccumulation and transformation of cadmium by Phaeodactylum tricornutum, Science in China Series B: Chemistry, 2009, 52(12): 2373-2380 下载全文
48 The determination of low-molecular-mass thiols with 4-(hydroxymercuric)benzoic acid as a tag using HPLC coupled online with UV/HCOOH-induced cold vapor generation AFS, Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2009, 877(28): 3428-3433 下载全文
47 Strategy for absolute quantification of proteins: CH3Hg+ labeling integrated molecular and elemental mass spectrometry, Journal of Analytical Atomic Spectrometry, 2009, 24(9): 1184-1187 下载全文
46 Different alkyl dimethacrylate mediated stearyl methacrylate monoliths for improving separation efficiency of typical alkylbenzenes and proteins, Journal of Chromatography A, 2009, 1216(15): 3098-3106 下载全文
45 In vivo phytochelatins and Hg-phytochelatin complexes in Hg-stressed Brassica chinensis L., Metallomics, 2009, 1(1): 101-106 下载全文
44 单叶新月蕨稀土富集能力与结合多肽结构研究, 中国稀土学报, 2009, 27(2): 289-296 下载全文
43 Quantification of selenium-tagged proteins in human plasma using species-unspecific isotope dilution ICP-DRC-qMS coupled on-line with anion exchange chromatography, Journal of Analytical Atomic Spectrometry, 2008, 23(11): 1545-1549 下载全文
42 Counting sulfhydryls and disulfide bonds in peptides and proteins using mercurial ions as an MS-tag, Journal of the American Society for Mass Spectrometry, 2008, 19(8): 1108-1113 下载全文
41 Modeling persistent organic pollutant (POP) partitioning between tree bark and air and its application to spatial monitoring of atmospheric POPs in mainland China, Environmental Science & Technology, 2008, 42(16): 6046-6051 下载全文
40 Alternative thermodiffusion interface for simultaneous speciation of organic and inorganic lead and mercury species by capillary GC-ICPMS using tri-n-propyl-lead chloride as an internal standard, Analytical Chemistry, 2008, 80(15): 6104-6109 下载全文
39 Synergistic defensive mechanism of phytochelatins and antioxidative enzymes in Brassica chinensis L. against Cd stress, Chinese Science Bulletin, 2008, 53(10): 1503-1511 下载全文
38 闽江水体镉的络合容量和配合物易变常数, 环境化学, 2008, 27(6): 795-799 下载全文
37 SEC-ICP-MS and ESI-MS/MS for analyzing in vitro and in vivo Cd-phytochelatin complexes in a Cd-hyperaccumulator Brassica chinensis, Journal of Analytical Atomic Spectrometry, 2007, 22(11): 1403-1408 下载全文
36 Pulsed large volume injection gas chromatography coupled with electron-capture negative ionization quadrupole mass spectrometry for simultaneous determination of typical halogenated persistent organic pollutants, Journal of the American Society for Mass Spectrometry, 2007, 18(8): 1375-1386 下载全文
35 A new vapor generation system for mercury species based on the UV irradiation of mercaptoethanol used in the determination of total and methyl mercury in environmental and biological samples by atomic fluorescence spectrometry, Analytical and Bioanalytical Chemistry, 2007, 388(4): 831-836 下载全文
34 Determination of cadmium in seawater by vapor generation atomic fluorescence spectrometry after online preconcentration with a novel alkyl phosphinic acid resin, Spectroscopy Letters, 2007, 40(3): 547-557 下载全文
33 On-line preconcentration with a novel alkyl phosphinic acid extraction resin coupled with inductively coupled plasma mass spectrometry for determination of trace rare earth elements in seawater, Talanta, 2007, 72(4): 1248-1254 下载全文
32 Vapour generation at a UV/TiO2 photocatalysis reaction device for determination and speciation of mercury by AFS and HPLC-AFS, Journal of Analytical Atomic Spectrometry, 2007, 22(3): 330-334 下载全文
31 Alternative normalization method of atmospheric polycyclic aromatic hydrocarbons pollution level recorded by tree bark, Environmental Science & Technology, 2006, 40(19): 5853-5859 下载全文
30 硒形态分析研究进展, 光谱学与光谱分析, 2006, 26(9): 1692-1701 下载全文
29 On-line pre-reduction of Se(VI) by thiourea for selenium speciation by hydride generation, Spectrochimica Acta Part B-Atomic Spectroscopy, 2006, 61(7): 803-809 下载全文
28 Subcellular distribution of rare earth elements and characterization of their binding species in a newly discovered hyperaccumulator Pronephrium simplex, Talanta, 2006, 70(1): 26-31 下载全文
27 Preparation and evaluation of stable coating for capillary electrophoresis using coupled chitosan as coated modifier, Talanta, 2006, 69(2): 463-468 下载全文
26 植物——实时富集大气持久性有机污染物的被动采样平台, 环境化学, 2005, 24(3): 233-240 下载全文
25 Chiral speciation and determination of DL-Selenomethionine enantiomers on a novel chiral ligand-exchange stationary phase, Analytical Sciences, 2005, 21(3): 253-257 下载全文
24 镧在菠菜叶绿体中的分布及其对光合作用的影响, 科学通报, 2005, 50(12): 1195-1200 下载全文
23 生物体系中硒代氨基酸的手性形态分析进展, 分析化学, 2005, 33(9): 1330-1334 下载全文
22 Preliminary study of the enrichment and fractionation of REEs in a newly discovered REE hyperaccumulator Pronephrium simplex by SEC-ICP-MS and MALDI-TOF/ESI-MS, Journal of Analytical Atomic Spectrometry, 2005, 20(8): 751-753 下载全文
21 用酰胺型键合固定相测定茶叶中嘌呤碱含量, 分析化学, 2005, 33(5): 627-630 下载全文
20 辛基型毛细管液相色谱整体柱的制备、表征及评价, 分析化学, 2005, 33(4): 467-470 下载全文
19 L-异亮氨酸型配体交换固定相对DL-氨基酸的拆分研究, 色谱, 2005, 23(2): 172-175 下载全文
18 Electrochemical vapor generation of selenium species after online photolysis and reduction by UV-irradiation under nano TiO2 photocatalysis and its application to selenium speciation by HPLC coupled with atomic fluorescence spectrometry, Analytical and Bioanalytical Chemistry, 2005, 381(2): 366-372 下载全文
17 Octyl-type monolithic columns of 530 mu m i.d. for capillary liquid chromatography, Journal of Chromatography A, 2005, 1062(2): 183-188 下载全文
16 Historical records of airborne polycyclic aromatic hydrocarbons by analyzing dated corks of the bark pocket in a Longpetiole Beech tree, Environmental Science & Technology, 2004, 38(18): 4739-4744 下载全文
15 Online pre-reduction of selenium(VI) with a newly designed UV/TiO2 photocatalysis reduction device, Journal of Analytical Atomic Spectrometry, 2004, 19(6): 715-716 下载全文
14 Concentrations of hazardous heavy metals in environmental samples collected in Xiamen, China, as determined by vapor generation non-dispersive atomic fluorescence spectrometry, Analytical Sciences, 2004, 20(1): 85-88 下载全文
13 Chromatographic behavior of cadmium in an ion-pair reversed-phase micro HPLC system and its application to the determination of bio-available cadmium in soil samples, Analytical and Bioanalytical Chemistry, 2003, 376(6): 923-927 下载全文
12 利用‘树皮'进行大气污染历史监测的新采样方法(英文), 环境化学, 2003, 22(3): 250-254 下载全文
11 高效液相色谱-原子荧光光谱联用中的连续数字信号采集和处理, 光谱学与光谱分析, 2003, 23(2): 354-357 下载全文
10 日本大学分析化学教育现状简介与借鉴, 大学化学, 2002, 17(5): 57-61 下载全文
9 环境样品中生物可利用态镉的反相离子对-HPLC-荧光检测, 高等学校化学学报, 2002, 23(9): 1692-1694 下载全文
8 Determination of Trace Impurities in High Purity Gallium by ICP-MS after Bis(1,1,3,3-tetramethylbutyl) dithiophosphinic Acid Extractive Separation, Analytical Sciences, 2001, 17: i1105-1108 下载全文
7 Speciation of rare earth elements in soil by sequential extraction then HPLC coupled with visible and ICP-MS detection, Fresenius Journal of Analytical Chemistry, 2001, 370(8): 1041-1047 下载全文
6 电化学氢化物发生法的进展及其在原子光谱分析中的应用, 分析化学, 2001, 29(8): 967-970 下载全文
5 Preliminary study of existing species of lanthanum in the spinach leaves after being cultivated with a culture solution containing lanthanum, Analytical Sciences, 2001, 17(6): 789-791 下载全文
4 Preliminary study on a vapor generation technique for nickel without using carbon monoxide by inductively coupled plasma atomic emission spectrometry, Spectrochimica Acta Part B-Atomic Spectroscopy, 2000, 55(7): 943-950 下载全文
3 软配位原子效应在锌和镉分离中的应用, 分析化学, 2000, 28(6): 759-763 下载全文
2 Evaluation of dissolved species of lanthanum in the solutions containing different amino acids by cation-exchange chromatography coupled with electrospray ionization mass spectrometry, Analytical Sciences, 2000, 16(2): 241-244 下载全文
1 Extracted species of rare earth elements using bis(1,1,3,3-tetramethylbutyl)phosphinic acid as an extractant and their effective mutual separation by high-performance extraction chromatography, Bulletin of the Chemical Society of Japan, 1999, 72(12): 2693-2697 下载全文

已申请专利

序号 专利名称 发明(设计)人 申请号 公开号 类型
18 手持式一体化液相色谱仪 张博,史晓慧,王晓兵,孙楷越,吕纪键 CN201810808694.X CN108593813A 发明专利
17 一种虹吸式毛细管色谱分离法 张博,高凡,周拙恒,朱珏,黄山青,林丽缘 CN201710443289.8 CN107037161A 发明专利
16 一种多探头液质联用点板装置 张博,丁芳芳,史晓慧,曾巨星,黄山青 CN201610216186.3 CN105891363A 发明专利
15 一种纳米填料毛细管色谱微柱制备装置及其制备方法 张博,王林,周拙恒,刘娅 CN201410310772.5 CN104034833B 发明专利
14 一种反相色谱硅胶填料的制备方法 张博,王林,丁芳芳,王鑫,叶淋泉,徐灵佳 CN201410326763.5 CN104069840B 发明专利
13 万伏高压电场增强液相色谱系统 张博,叶淋泉,高凡,徐灵佳 CN201410154755.7 CN103884814B 发明专利
12 基于液滴微流控技术制备粒径均一可控色谱填料的方法 张博,王鑫,丁芳芳,王林,周拙恒 CN201410084209.0 CN103816881B 发明专利
11 一种基体辅助激光解吸离子源质谱的在线样品制备装置 张博,肖志良,王雪 CN201210086514.4 CN102621253B 发明专利
10 一种毛细管固相萃取微柱的制备方法 张博,肖志良,牛萌蕾 CN201110416485.9 CN102513073B 发明专利
9 用于蛋白质二维电泳分离的微流控芯片接口的制备方法 宋站雨,王秋泉,张博,陈宏 CN201110343112.3 CN102504010A 发明专利
8 可调式即时封装树木表皮采样器 王秋泉,张天闻,赵玉丽 CN200910112192.4 CN101608980 发明专利
7 应用于连续流动体系中的气液分离器
邱建华,王秋泉 CN200620121485.0 CN200948374 实用新型专利
6 便携式液相色谱仪 王秋泉,付强,徐振东 CN200610055109.0 CN1815221 发明专利
5 用于便携式液相色谱仪检测系统的光纤组件 付强,王秋泉,徐振东 CN200620006301.6 CN2874495 实用新型专利
4 紫外-可见吸收/荧光两用流通池 付强,王秋泉,罗世鹏,刘永玉 CN200610009153.8 CN1815218 发明专利
3 可调式色谱微柱—流通池安装架 徐振东,付强,王秋泉 CN200620005095.7 CN2874494 实用新型专利
2 十八烷基型整体式液相色谱微柱的制备方法 徐振东,王秋泉,付强 CN200610054640.6 CN1833772 发明专利
1 辛基型微柱液相色谱整体柱的制备方法 黄晓佳,黄本立,王秋泉 CN200310121890.3 CN1554945 发明专利