Title:    Mapping Biomolecular Interaction at Scale with Protein Cellular Biophysics and Protein Mass Spectrometry

Time:     2025-06-18 16:00

Lecturer:  Chris Soon Heng TAN

Southern University of Science and Technology

Venue:    Room 202, Lu-Jiaxi Building

Abstract：

The interaction of proteins with metabolites and other proteins underlies most if not all cellular activities, while many bioactive xenobiotics perturb cellular processes through direct physical interactions with proteins. Mapping these biomolecular interactions can help unravel the complex protein machines and molecular pathways underlying various cellular processes, and unveil wiring of the cells that are dysregulated in diseases and perturbed by chemicals. Nevertheless, there are currently limited time- and cost-effective techniques for profiling intracellular protein-protein and protein-chemical interactions at scale and in situ. My laboratory integrates methods in computational sciences, analytical chemistry and molecular biology with new cellular protein biophysics techniques for large-scale profiling of biomolecular interactions. Tedious chemical derivatization and genetic engineering are avoided which facilitate rapid deployment of our methods for systematic investigation of various biological phenomena including deciphering the mechanism-of-action of bioactive chemicals and therapeutic agents. I will describe these methods developed in my laboratory, as well as our recent findings deploying them for proteome-wide screens for proteins and their interactions perturbed by microproteins, natural products and FDA approved drugs.