Lanthanide-Coded Protease-Specific Peptide-Nanoparticle Probes for a Label-Free Multiplex Protease Assay Using Element Mass Spectrometry: A Proof-of-Concept Study

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION , 50 , 22, 5130-5133, Publication Date : 2011

Author: Xiaowen Yan, Limin Yang, and Qiuquan Wang*

Abstract：

Coded messages: Lanthanide (Ln) coded protease-specific peptide–nanoparticle (NPs) conjugates act as probes for a multiplex protease assay (MPA) using inductively coupled plasma mass spectrometry (ICP-MS; see picture). MPA can be achieved by centrifugal separation of the protease-cleaved and uncleaved probes and subsequent determination of the released Ln tags.

http://onlinelibrary.wiley.com/doi/10.1002/anie.201101087/pdf